Proteomic difference of serum containing Cinnamon Twig Decoction Plus Pueraria on annulus fibrosus cells

doi:10.3969/j.issn.2095-4344.2014.11.013

Abstract

Abstract:

BACKGROUND: Anulus fibrosus cleavage is the main contributing factor of intervertebral disk degeneration in cervical spondylosis. Previous studies of our research group have demonstrated that, Cinnamon Twig Decoction Plus Pueraria is a definitely effective treatment of cervical spondylosis. However, differential proteomic research addressing the intervention of Cinnamon Twig Decoction Plus Pueraria on anulus fibrosus cells is rarely reported.

OBJECTIVE: To explore the target or related proteins of Cinnamon Twig Decoction Plus Pueraria intervention on anulus fibrosus cells, at the differential proteomics level.

METHODS: Anulus fibrosus cells were collected from Sprague-Dawley rats that were cultured in vitro. Cultivated anulus fibrosus cells at passage 3 were randomly divided into two groups: experiment group was cultured in serum containing Cinnamon Twig Decoction Plus Pueraria, while control group in saline. The isotopically labeled relative and absolute quantification was applied to mark the sample proteins in two groups. Two-dimensional liquid chromatography mass spectrometry was used to identify the proteins and relative quantitative analysis was performed.
RESULTS AND CONCLUSION: Compared with the control group, platelet-derived growth factor subunit A, bone morphogenetic protein 2, insulin-like growth factor I, aggrecan core protein, transforming growth factor beta-3, interleukin-1 beta expression were increased in the experiment group, while fibroblast growth factor 2 and collagen alpha-1 chain expression was decreased. Cinnamon Twig Decoction Plus Pueraria can repair the degeneration of anulus fibrosus cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: cervical vertebrae, intervertebral disk, drugs, Chinese herbal, serum, proteomics

CLC Number:

R318

Zhong Wei-hong, Li Yu-tao, Zheng Qi-kai, Ye Jia-jia, Lin Jian-ping, Wang Shi-zhong. Proteomic difference of serum containing Cinnamon Twig Decoction Plus Pueraria on annulus fibrosus cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(11): 1718-1723.

Figures/Tables 2

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